![Cell growth and morphological suppression of the cdc12-6 septin mutant by overexpression of Bni5-N-GFP, but not Bni5-C-GFP. Related to Fig. 2. (A) Effects of overexpression of different BNI5 alleles on the temperature sensitivity of cdc12-6 cells. Cells were grown in methionine-depleted medium to induce BNI5 gene expression. Strains used: YEF11362 (cdc12-6 bni5Δ [BNI5]), YEF11363 (cdc12-6 bni5Δ [BNI5-C-GFP]), YEF11312 (cdc12-6 bni5Δ [BNI5-N-GFP]), and YEF11313 (cdc12-6 bni5Δ [GFP]). (B) Representative images of cdc12-6 cells with induced expression of different BNI5 alleles. Cells were grown in SC-Ura-Met liquid medium to induce their expression at 25°C or 32°C for 16 h before imaging. Strains used are the same as in A.](https://cdn.rupress.org/rup/content_public/journal/jcb/224/12/10.1083_jcb.202311040/1/jcb_202311040_figs1.png?Expires=1767658332&Signature=12iGeAMZmfXQHfWMswSHwNm-NKdPsl0ziRa2Hgu9rZmWLnxHhgKKEPOqpUKRRYmgkFKMi-gtAtunUrd4pHZp9WM4cstnYQ6-DUJzAO9YB9IZsG7cfnS~D9o1wKsDs2d-KVhk4D09KEP-jL0q4DasO9J5vnj--Lo6Zc-y-ZcLaR~ujVN6T6ep~l2~vQpHx2~Uqx6-fuONbPt4vqocZdjAVT-yBCnVy9HZ5lfqv7cLmM-NHCn24HE5rlF-3EEQYf4wfZ4pBqTAfetQmMVZP8fMZHHHhEqWZPMdYWKpCmT12VecwJHwRrhyACM4Ag~H1iGWj168WdhgTU86vMYu3CMQ4Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure S1.
Cell growth and morphological suppression of the cdc12-6 septin mutant by overexpression of Bni5-N-GFP, but not Bni5-C-GFP. Related to Fig. 2. (A) Effects of overexpression of different BNI5 alleles on the temperature sensitivity of cdc12-6 cells. Cells were grown in methionine-depleted medium to induce BNI5 gene expression. Strains used: YEF11362 (cdc12-6 bni5Δ [BNI5]), YEF11363 (cdc12-6 bni5Δ [BNI5-C-GFP]), YEF11312 (cdc12-6 bni5Δ [BNI5-N-GFP]), and YEF11313 (cdc12-6 bni5Δ [GFP]). (B) Representative images of cdc12-6 cells with induced expression of different BNI5 alleles. Cells were grown in SC-Ura-Met liquid medium to induce their expression at 25°C or 32°C for 16 h before imaging. Strains used are the same as in A.