scRNA-seq of LECs derived from human skin and adipose tissue reveals the presence of seven subsets of LECs. (A) Schematic overview of LEC isolation process from human skin or adipose tissue for scRNA-seq (seven donors). (B) Representative flow cytometry plot of the isolated frozen stromal vascular fraction from skin and adipose tissue showing a population of LECs (CD45⁻CD31⁺PDPN⁺). The full gating scheme can be found in Fig. S2, A and B. (C) Seven LEC clusters from human skin or adipose tissue visualized in a UMAP plot. (D) Schematic of a LV depicting the different subsets of LECs as detected in the scRNA-seq data. BM: basement membrane. (E) Representation of the proportions and percentages of each LEC subset present in the skin and adipose tissue. The adjusted P value (P_adj) of the differential abundance of LEC clusters between both tissue types was computed using the Bioconductor/R edge R package. Collector LECs were significantly more abundant in adipose tissue compared with skin (P_adj < 0.05). (F) Bubble plot showing the expression of selected pan-EC, pan-LEC, and LEC subset marker genes in the seven subsets. Expression data from the skin and adipose tissue combined are shown. The color and size of each dot represent the expression level and cell fraction of the indicated genes, respectively. (G) UMAP plots showing the expression of specific LEC marker genes, i.e., PROX1, PDPN, LYVE1, CCL21. Abbreviations: cap: capillary; precoll: pre-collector; coll: collector; prolif: proliferative.