LVs are more abundant in the skin and adjacent s.c. adipose tissue than in the deeper adipose tissue. (A) aSMA⁺ LYVE-1⁺ lymphatic collectors in adipose tissue (depicted by arrows). Only two lymphatic collectors were observed in this set of images. Scale bar: 100 µm. (B) Representative examples of spectrally unmixed high-power field images for sections of S&AT, as well as dAT. Scale bars: 100 µm. (C) Corresponding automated tissue segmentation results, highlighting the detection of LVs, BVs, and the surrounding tissue (stroma). LVs in B and C are depicted by an arrow. Scale bars: 100 µm. (D and E) Quantification of the (D) mean area (µm²) or number of LVs and (E) BVs for n = 3 donors. One dot represents the mean of the quantification of all images for one donor per tissue type. Data from the same donor are connected by a line and analyzed by paired Student’s t test. *P < 0.05. (F and G) Quantification of the (F) mean area (µm²) or (G) number of LVs. Mean and standard deviation are shown separately for each donor (one dot represents one image). For each tissue, sections of three different depths were evaluated. Images were acquired by multispectral microscopy from a representative set of S&AT and dAT regions (12–47 images per tissue and patients, total of 451 images). S&AT, human skin and attached s.c. adipose tissue; dAT, deeper s.c. adipose tissue.