Membrane thickness variations between functionally distinct membranes within the same organelle. (A–C) An analysis of the chloroplast membrane system. (A) Central slice from C. reinhardtii tomogram (Kelley et al., 2024, Preprint) (EMPIAR-11830, 573.mrc, 7.84 Å/pixel at bin4), showing the thylakoid, inner chloroplast membranes (Chl IMs), and Chl OMs. Scale bar, 100 nm. (B) Thickness-mapped segmentation of the chloroplast, where color values indicate mean membrane thickness. (C) Comparative thickness distributions for Chl OM, Chl IM, and the thylakoid. Box plots show median (center line), interquartile range (box), and whiskers extending to 1.5× the interquartile range. (D–F) Analysis of the Golgi apparatus membranes. (D) Central slice from C. reinhardtii tomogram (Kelley et al., 2024, Preprint) (EMPIAR-11830, 1570.mrc, 7.84 Å/pixel at bin4), showing the ER and Golgi cisternae from the CGN through the medial Golgi stack to TGN. Scale bar, 100 nm. (E) Thickness-mapped segmentation of the membranes, where color values indicate mean membrane thickness. (F) Thickness distribution analysis across the Golgi stack highlights distinct cisternae populations within the Golgi apparatus, with membrane thickness progressing from ER-like values in the CGN, decreasing in the medial cisternae, and then increasing toward the TGN. Box plots show median (center line), interquartile range (box), and whiskers extending to 1.5× the interquartile range.
Figure 7.

Membrane thickness variations between functionally distinct membranes within the same organelle. (A–C) An analysis of the chloroplast membrane system. (A) Central slice from C. reinhardtii tomogram (Kelley et al., 2024, Preprint) (EMPIAR-11830, 573.mrc, 7.84 Å/pixel at bin4), showing the thylakoid, inner chloroplast membranes (Chl IMs), and Chl OMs. Scale bar, 100 nm. (B) Thickness-mapped segmentation of the chloroplast, where color values indicate mean membrane thickness. (C) Comparative thickness distributions for Chl OM, Chl IM, and the thylakoid. Box plots show median (center line), interquartile range (box), and whiskers extending to 1.5× the interquartile range. (D–F) Analysis of the Golgi apparatus membranes. (D) Central slice from C. reinhardtii tomogram (Kelley et al., 2024, Preprint) (EMPIAR-11830, 1570.mrc, 7.84 Å/pixel at bin4), showing the ER and Golgi cisternae from the CGN through the medial Golgi stack to TGN. Scale bar, 100 nm. (E) Thickness-mapped segmentation of the membranes, where color values indicate mean membrane thickness. (F) Thickness distribution analysis across the Golgi stack highlights distinct cisternae populations within the Golgi apparatus, with membrane thickness progressing from ER-like values in the CGN, decreasing in the medial cisternae, and then increasing toward the TGN. Box plots show median (center line), interquartile range (box), and whiskers extending to 1.5× the interquartile range.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal