Contractile parameters in response to MT network manipulation in muscle fibers change during long-term ex vivoculture. (A–C) Quantification of contraction, relaxation duration, and relaxation velocity measurements of muscle fibers cultured in 2D and 3D at day 1, treated with nocodazole or taxol, or untreated (control). (D–F) Quantification of contraction, relaxation duration, and relaxation velocity measurements of muscle fibers cultured in 2D and 3D at day 7, treated with nocodazole or taxol, or untreated (control). Data are means ± SEM; large dots represent mean values per mouse, and small dots represent single muscle fibers. Significance was determined using one-way ANOVA within the culture condition (2D or 3D), with P < 0.05 considered as significant.
Figure S7.

Contractile parameters in response to MT network manipulation in muscle fibers change during long-term ex vivo culture . (A–C) Quantification of contraction, relaxation duration, and relaxation velocity measurements of muscle fibers cultured in 2D and 3D at day 1, treated with nocodazole or taxol, or untreated (control). (D–F) Quantification of contraction, relaxation duration, and relaxation velocity measurements of muscle fibers cultured in 2D and 3D at day 7, treated with nocodazole or taxol, or untreated (control). Data are means ± SEM; large dots represent mean values per mouse, and small dots represent single muscle fibers. Significance was determined using one-way ANOVA within the culture condition (2D or 3D), with P < 0.05 considered as significant.

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