MT networks in muscle fibers change during long-term ex vivoculture, thereby impairing sarcomere shortening. (A) Representative confocal maximum intensity projection images taken with a 60× objective stained for showing α-tubulin (grey) and nuclei (blue) in muscle fibers cultured for 1 and 7 days in 2D and 3D. (B–D) Quantification of contractile measurements of muscle fibers cultured in 2D and 3D at day 1, treated with nocodazole, taxol, or untreated (control). (E–G) Quantification of contractile measurements of muscle fibers cultured in 2D and 3D at day 7, treated with nocodazole, taxol, or untreated (control). Data are means ± SEM; large dots represent mean values per mouse, and small dots represent single muscle fibers. Significance was determined using one-way ANOVA with P < 0.05 considered as significant with * = P < 0.05.
Figure 5.

MT networks in muscle fibers change during long-term ex vivo culture , thereby impairing sarcomere shortening. (A) Representative confocal maximum intensity projection images taken with a 60× objective stained for showing α-tubulin (grey) and nuclei (blue) in muscle fibers cultured for 1 and 7 days in 2D and 3D. (B–D) Quantification of contractile measurements of muscle fibers cultured in 2D and 3D at day 1, treated with nocodazole, taxol, or untreated (control). (E–G) Quantification of contractile measurements of muscle fibers cultured in 2D and 3D at day 7, treated with nocodazole, taxol, or untreated (control). Data are means ± SEM; large dots represent mean values per mouse, and small dots represent single muscle fibers. Significance was determined using one-way ANOVA with P < 0.05 considered as significant with * = P < 0.05.

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