PARI promotes actin-patch clearance at the intracellular bridge in cells with chromatin bridges. (A) Time-lapse images of HeLa cells expressing H2B-mCherry (magenta) and GFP-actin (green), treated with either DMSO or ICRF-193, and transfected with siCtrl or siPARI-1. Images show time relative to anaphase onset (t = 0). Scale bar: 5 μm. Arrows point to actin patches, arrowheads point to chromatin bridges, and asterisks mark actin-patch clearance. Movies corresponding to the cells shown are provided as Videos 8, 9, 10, and 11. (B) Actin-cluster index plotted for five representative cells per condition. Asterisks mark traces of cells shown in A. (C) Population-level analysis showing the cumulative percentage of cells that have cleared actin clusters from the ICB over time after anaphase onset. n = cells pooled from N = 3 independent experiments with similar results. siCtrl +DMSO vs. siCtrl +ICRF-193, P < 0.0001; siCtrl +ICRF-193 vs. siPARI-1 +ICRF-193, P < 0.0001, Mann–Whitney test.