PARI does not stabilize the midbody upon NPC defects. (A) Western blot showing partial depletion of NUP153. Membrane probed with a NUP153 antibody and alpha-tubulin as a loading control. (B) Cells transfected with siCtrl, siNUP153, siPARI-1, and a combination of siPARI-1 + siNUP153. Cells were synchronized with thymidine for 24 h, released for 16 h, and fixed. Scale bars: 10 µm. (C) Quantification of the fraction of cells showing midbodies (% cytokinesis). Mean and SD are shown. Student’s paired t test, P > 0.05 = ns (n > 789, N = 3). NPC, nuclear pore complex. Source data are available for this figure: SourceData F8.