PARI depletion stabilizes chromatin bridges during interphase but does not lead to cytokinesis failure. (A) Time-lapse imaging of HeLa cells expressing eGFP-BAF (green) and stained with SiR-tubulin (magenta) to visualize chromatin bridges and midbody microtubules. Three representative divisions are shown: siCtrl+DMSO without bridge (top), siCtrl+DMSO with a spontaneous chromatin bridge (middle), and siCtrl+ICRF-193 with a persistent chromatin bridge (bottom). White arrows indicate chromatin bridges; asterisks indicate midbody disassembly. Time in minutes after midbody formation. Scale bar: 5 μm. Movies corresponding to the cells shown are provided as Videos 5, 6, and 7. (B) Quantification of cells with visible chromatin bridges in the indicated conditions. n = number of cells pooled from two independent experiments with similar results. (C and D) Time from midbody formation to disassembly in cells under the indicated conditions. Each circle represents one cell, pooled from two independent experiments; triangles indicate the mean value of each experiment; bars indicate median ± IQR. ns: not significant; P-values from the Mann–Whitney test. (E) Plots showing the time from midbody formation to chromatin bridge resolution for cells with bridges. n = number of cells pooled from two independent experiments with similar results. P < 0.0005, Mann–Whitney test for both DMSO and ICRF-193. (F) Frequency of binucleation in cells with chromatin bridges under the indicated conditions. ns, nonsignificant (P > 0.05, Fisher’s exact test).