Deletion of the PCNA unloader Elg1 is sufficient for abscission inhibition in response to dicentric bridges. (A) Schematic illustrating the generation of the conditional dicentric strain (left) and the formation of chromosome bridges upon biorientation of kinetochores (right). Metaphase and anaphase cells with or without dicentric bridges are shown on the right. Green dots: spindle poles; black dots: kinetochores; orange lines: kinetochore microtubules; green lines: dicentric chromosomes. (B) Abscission dynamics in dicentric strains of the indicated genotypes. Dicentric bridges were imaged with Htb2-mCherry, and abscission was scored with GFP-CAAX. ELG1 cells with bridge vs. elg1∆ with bridge, P < 0.0001, Mann–Whitney test. n = number of cells pooled from two (B, ELG1) or three (B, elg1∆) independent time-lapse experiments.