Timing of chromatin bridge resolution, membrane ingression, and abscission in WT and srs2Δ cells. (A) Schematic representation of key cell division events, including chromatin bridge resolution, membrane ingression, and abscission. The time intervals measured in the analysis are indicated by horizontal arrows. The nucleus is in red, the spindle pole bodies are black circles, and the plasma membrane is in gray. (B) Cumulative frequency plots showing the timing of chromatin bridge resolution (top), membrane ingression (middle), and abscission (bottom) in WT and srs2Δ cells, with or without HU treatment. The number of cells analyzed for each condition is indicated in the legend. Time from anaphase onset was defined by nuclear elongation (Htb2-mCherry) in the top panel, and by spindle elongation (Spc42-GFP) in the middle and bottom panels. Data in the top panel are replotted from Fig. 1, A and B; data in the middle and bottom panels are from Fig. 2, B and C. (C) Fraction of cells of the indicated strains undergoing chromosome segregation with RPA foci at 37°C (top) and fraction of cells that segregated with RPA foci and had RPA-coated chromatin bridge (bottom). Cells are pooled from two independent experiments; P-values correspond to Fisher’s exact test.