Chromosome segregation (Htb2-mCherry) and RPA focus formation (Rfa2-GFP) in cells of the indicated strains, shown in Fig. 1 A , including all time points, with and without previous exposure to HU. Only cells with RPA foci are shown. Yellow arrows indicate chromatin bridges, green arrows point toward RPA foci, white arrowheads mark RPA bridges, and asterisks note bridge resolution. Scale bar: 5 μm.