Srs2 promotes chromatin bridge resolution. (A) Chromosome segregation (Htb2-mCherry) and RPA foci formation (Rfa2-GFP) in cells of the indicated strains with and without previous exposure to HU. Selected frames are shown, and images are acquired every 2 min (all frames are shown in Fig. S1). Only cells with RPA foci are shown. Yellow arrows indicate chromatin bridges, green arrows point toward RPA foci, white arrowheads mark RPA bridges, and asterisks note bridge resolution. Scale bar: 5 μm. (B) Time of bridge resolution (Htb2-mCherry) from the time of anaphase onset, defined by the rapid elongation of the nucleus. n = number of cells pooled from two independent experiments with similar results. (C) Fraction of cells in B undergoing chromosome segregation with RPA foci. (D) Fraction of cells in B, which segregated with RPA foci and had RPA-coated chromatin bridge. (E) Same data as in B but distinguishing between cells containing vs. not containing RPA anaphase foci. The P values correspond to Dunn’s multiple comparison test (B), Fisher’s exact test (C and D), and Mann–Whitney test (E).