Reduced cytokine production by CD4 ⁺ and CD8 ⁺ T cells of the patient with ORAI1 mutation. (A) Flow cytometry analysis of CD27 and CD45RA expression on CD8⁺ T cells within PBMC of an HD control and the patient. (B) Flow cytometry analysis of IFN-γ production and T-bet expression of CD4⁺ T cells within PBMC from an HD and the patient. Cells were stimulated with PMA/ionomycin for 5 h. (C) TNF production in in vitro–expanded T cells from the patient (Pat) and his mother (Mo) stimulated with PMA/ionomycin for 4 h. Representative flow cytometry plot (left) and quantification (right). For CD4⁺ T cells: P = 0.006. For CD8⁺ T cells: P = 0.0016. (D) GM-CSF production in in vitro–expanded T cells from the patient (Pat) and his mother (Mo) stimulated with PMA/ionomycin for 4 h. Representative flow cytometry plot (left) and quantification (right). For CD4⁺ T cells: P = 0.0091. For CD8⁺ T cells: P = 0.0003. (E) IL-17 production in in vitro–expanded CD4⁺ T cells from the HD, the patient (Pat), his father (Fa), and his mother (Mo) stimulated with PMA/ionomycin for 4 h (representative flow cytometry plots are shown in Fig. 4 F). P = 0.0212; 0.0011; 0.0001. Data in C–E are from three to four independent experiments for CD4⁺ and CD8⁺ T cells, respectively. Statistical significance was calculated using an unpaired t test in C and D and ordinary one-way ANOVA in E. *P < 0.05, **P < 0.01, ***P < 0.001.