Strong SOCE defects in T cells of ORAI1 and STIM1 mutant patients. (A and B) Calcium measurements in in vitro–expanded T cells from the ORAI1 mutant patient, his heterozygous mother, a patient with STIM1 LOF mutation (STIM1 c.497+776A>G), and his heterozygous mother using Fura-2 AM. Representative time course of Fura-2 fluorescence ratios (340/380 nm) over time (left). Quantification of the AUC and slope of Ca²⁺ influx (right). Data are from at least three independent experiments. (A) Cells were placed in 0 mM Ca²⁺ Ringer’s solution, and SOCE was induced by anti-CD3 cross-linking, followed by the addition of 1 mM Ca²⁺ Ringer’s solution, and stimulation with 1 µM ionomycin (iono). For anti-CD3 AUC: P = 0.9991; <0.0001; 0.0049; 0.9774. For anti-CD3 slope: P = 0.7349; 0.0051; 0.0017; 0.9838. For iono AUC: P = 0.9997; <0.0001; <0.0001; 0.3427. For iono slope: P = 0.9685; <0.0001; <0.0001; 0.1042. (B) Cells were placed in 0 mM Ca²⁺ Ringer’s solution, and SOCE was induced by stimulation with 1 µM TG followed by the addition of 1 mM Ca²⁺. AUC: P = 0.9633; <0.0001; <0.0001; 0.7556. Slope: P = 0.7963; <0.0001; <0.0001; 0.4852. Statistical significance was calculated using ordinary one-way ANOVA. ns, not significant, **P < 0.01, ***P < 0.001. AUC, area under the curve.