Figure 3.
TRAC splicing and transcripts activity. (A) Schematic representation of the artificial TCRα gene created to study the splicing between exons 2, 3, and 4 of TRAC. The two mutations tested are depicted together with the various splice variants. STOP*: noncanonical STOP codons generated by alternative splicing. (B and C) TCRα-deficient Jurkat cells transduced with EV or a plasmid containing an artificial TCRα gene described in A encoding the WT or the indicated variant of TRAC in TCRα. (B) Cell surface expression of CD3ε and TCRβ evaluated by flow cytometry. (C) RT-PCR analysis of TRAC with primers binding to exon 2 (forward; for) and intron 3 (reverse; rev). NT: Non-transduced. The data shown are representative of three independent experiments. Source data are available for this figure: SourceData F3.

TRAC splicing and transcripts activity. (A) Schematic representation of the artificial TCRα gene created to study the splicing between exons 2, 3, and 4 of TRAC. The two mutations tested are depicted together with the various splice variants. STOP*: noncanonical STOP codons generated by alternative splicing. (B and C) TCRα-deficient Jurkat cells transduced with EV or a plasmid containing an artificial TCRα gene described in A encoding the WT or the indicated variant of TRAC in TCRα. (B) Cell surface expression of CD3ε and TCRβ evaluated by flow cytometry. (C) RT-PCR analysis of TRAC with primers binding to exon 2 (forward; for) and intron 3 (reverse; rev). NT: Non-transduced. The data shown are representative of three independent experiments. Source data are available for this figure: SourceData F3.

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