(Related to Fig. 3 ). (A) Representative immunofluorescence images showing activated ERM (P-ERM) at TNTs in hOC. F-actin (phalloidin, magenta), nuclei (DAPI, cyan), and P-ERM (green). Scale bar, 10 µm. See also Video 7. (B) Representative microscopy images (F-actin, phalloidin, gray) illustrating the increase of TNT number after moesin depletion: (left) moesin KO versus CTL mOC and (right) siMoesin versus siCTL hOC. Scale bars, (left) 100 µm and (right) 50 µm. (C) Representative image of a 1:1 mixed culture of mOC control (CTL, transduced with mCherry-lifeact) and moesin KO (transduced with GFP-lifeact) seeded on glass coverslides on day 3 of differentiation. Arrowheads show TNT-like protrusions. Scale bar, 20 µm.