(Related to Fig. 2 ). (A and B) Representative western blot analysis of the level of ERM during murine (A, mOC) and human (B, hOC) osteoclast differentiation, actin was used as loading control. (C). Representative western blot analysis of the level of ERM and activated ERM (P-ERM) in the three individual KO mOC. The quantification of the expression level, normalized to actin, is shown under each band. (D) Quantification of fusion index in control (CTL) and ezrin and radixin KO mOC. Each circle represents an independent experiment, n = 4, SDs are shown. (E) Quantification of the area occupied by osteoclasts in control (CTL) versus moesin KO (MKO) mOC after microscopy analysis. Each circle represents an independent experiment, n = 6. (F) Flow cytometry analysis of the percentage of β3-integrin-positive cells in control (CTL) versus moesin KO (MKO) mOC. Each circle represents an independent experiment, n = 6, SDs are shown. (G) Quantification of mRNA expression of genes overexpressed in osteoclasts measured by RT-PCR in control (CTL, blue) versus moesin KO mOC (orange) on days 3, 5, and 7 of differentiation. Actin mRNA level was used as control. Each circle represents an independent experiment, n = 3 independent experiments, SDs are shown. (H and I) Western blot analysis of Scr (H) and cathepsin K (I). (left) Representative experiment and (right) quantification of expression level normalized to actin. Each circle represents a single donor, n = 3. Predicted molecular weight are indicated on western blots. Actin panel is the same in H and I. Statistical analyses: (D) Kruskal–Wallis and then Dunn’s multiple comparison tests. n.s., not significant. Source data are available for this figure: SourceData FS1.