AKT activates lysosomal TMEM175. (A and B) Lysosomal currents were recorded from TMEM175-transfected HEK293T cells with (B) or without (A) starvation with pipette solution containing 50 mM K⁺, pH 4.6. Left: Representative I-Ψ relationships of currents recorded from the same lysosome with bath containing 0 mM K⁺ (NMDG), 150 mM K⁺, or 150 mM K⁺ together with SC79, an AKT activator (10 µM). Middle and right panels: Summary of the inward (at −100 mV) and outward (at +100 mV) current amplitudes. Numbers of lysosomal patches in both fed (A) and starved (B) are n = 5. Error bars are SEM.