Lysosomal pH of WT and TMEM175 KO cells by mouse pair. (A–C) Lysosomal pH measurement of cultured mouse hippocampal neurons (A), midbrain neurons (B), and extracted peritoneal macrophages (C) from WT and TMEM175 KO mice. Each cell culture had at least three pairs of WT and TMEM175 KO mice from at least two litters. Each circle represents the lysosomal pH average of all the cells analyzed from one mouse in both the nutrient-replete and depleted conditions. Error bars are SEM.