Phosphoproteomics reveals cell cycle, cytoskeleton, and translation as the main processes regulated by Mos–MAPK. (A) Volcano plot of the quantified phosphopeptides. The majority of phosphopeptides were manually classified into four categories: translation, cytoskeleton, cell cycle regulation, and MAPK pathway. The rest of the phosphopeptides, including those from unidentified proteins, are marked as gray dots. For the complete dataset, see Datas S1 and S3. (B) Functional modules were manually assembled from the list of significantly affected phosphopeptides. Color code indicates the average log₂ fold change in phosphopeptide abundance upon Mos–MAPK inhibition. The numerical data are shown in a tabulated form on Table S1.