Homology searches of adaptor complexes AP1 and AP4 components, epsin, and tepsin across 53 pan-eukaryotic species with statistical assessment for co-occurrence of AP4 and tepsin. (A) Coulson plot representing result of HMMer search based comparative genomics conducted across selected eukaryotic species with their eukaryotic classification. Circles or sections filled with color demonstrate presence of protein homologs; unfilled sections demonstrate loss. Color only represents single paralog identification; numbers represent multiple paralogs. Key is provided for the abbreviated species binomial names. (B) Independence chi-square test calculation matrices for presence of absence of variables: AP4 and tepsin. First matrix shows actual values of different variations of presence or absence of both the variables, second matrix shows the expected frequencies for the four variations, and third matrix represents the chi-square points, along with the calculated χ² and P value. (C) Graphical representation of the AP4 and tepsin co-occurrence test is provided with the calculated χ² and P value, along with the blocks of graph representing all the variations of occurrence.