Biotinylated proteins identified by mass spectrometry. (A and B) show the difference in protein hits between WT (WT30) and TgTEP-TurboID (S30) sample following 30 min of biotinylation, whereas (B) shows the difference between WT (WT6) and TurboID (S6) sample following 6 h of biotinylation. (C) shows the total number of proteins enriched during the 6-h experiment (S6) vs the 30-min experiment (S30) after normalization of protein abundance. Proteins of particular interest in panels A–C are numerated and listed in the table in D. (E) Halo-tagged SAG1 was labelled with a cell non-permeable dye for an hour, then parasites were allowed to replicate for a further 24 h to observe endocytosis of SAG1-containing vesicles. After addition of rapamycin, endocytosis was not affected. (F) Quantification of endocytosis events is similar in non-induced parasites (-Rapa) or induced parasites (+48 Rapa). (G) Quantification of vesicles demonstrates accumulation and enlargement upon deletion of TgTEP. Scale bars are 5 μm. Data are plotted as mean ± SD. Unpaired two-tailed Student’s t test was calculated for F and G, where P < 0.0001.