TgTEP localizes close to the trans-Golgi. Colocalization analysis of TgTEP (in magenta) with various markers (in yellow) done in triplicate with a minimum of 100 parasites observed per replicate. Profile plots were taken across the respective lines. (A and B) Parasites expressing GRASP-RFP (a cis-Golgi marker) show no clear overlap with TgTEP. (C and D) Parasites expressing SortLR-Halo (a marker for the trans-Golgi) show a partial overlap with TgTEP. (E–J) Similarly, colocalization analysis with other postGolgi markers, such as parasites expressing syntaxin-6-Halo (E and F), stained with α-DrpB (G and H) or α-ProM2AP (I and J) show only a partial overlap with TgTEP. (K and L) Colocalization analysis on extracellular parasites between TgTEP and the PLVAC marker CPL shows no significant overlap. Scale bars: 5 µm (2 µm for CPL). Prior to obtaining the profile plots, images were converted to 8 bit for intensity normalization.
Figure 2.

TgTEP localizes close to the trans-Golgi. Colocalization analysis of TgTEP (in magenta) with various markers (in yellow) done in triplicate with a minimum of 100 parasites observed per replicate. Profile plots were taken across the respective lines. (A and B) Parasites expressing GRASP-RFP (a cis-Golgi marker) show no clear overlap with TgTEP. (C and D) Parasites expressing SortLR-Halo (a marker for the trans-Golgi) show a partial overlap with TgTEP. (E–J) Similarly, colocalization analysis with other postGolgi markers, such as parasites expressing syntaxin-6-Halo (E and F), stained with α-DrpB (G and H) or α-ProM2AP (I and J) show only a partial overlap with TgTEP. (K and L) Colocalization analysis on extracellular parasites between TgTEP and the PLVAC marker CPL shows no significant overlap. Scale bars: 5 µm (2 µm for CPL). Prior to obtaining the profile plots, images were converted to 8 bit for intensity normalization.

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