Evolution and structural assessment of adaptor complexes and accessory components in Apicomplexa. (A) Schematic of TgTEP protein architecture showing an ENTH_VHS domain and disordered regions (marked “D”), with no additional annotated motifs. Generated using DOG 2.0 (Ren et al., 2009). (B) Coulson plot representing the result of HMMer searches in 10 selected apicomplexans. Colored areas represent presence of protein homologs, white sections represent absence or unidentified, and numbers represent multiple paralogs identified. Respective proteins are annotated along with the binomial names of the species. (C) Phylogenetic analysis of epsin and tepsin using human protein sequences as references. aBayes represents Bayesian posterior probability calculations; NP represents nonparametric bootstrap support values. Clade in green represents tepsin; clade in blue represents epsin. (D) 3D superimposition of predicted AP4-ε structures of human (red) and T. gondii (yellow), TM-score, and pLDDT are provided. (E) 3D superimposition of the predicted TgTEP (green) with the crystal of human tepsin ENTH domain (magenta), TM-score, and pLDDT are provided.