Impact of OPTN-GFP expression on organelle morphology in HEK-293 cells. (A) MitoTracker Red CM-H2Xros (MT) and ER-Tracker Blue-White DPX (ER) staining. (B) LysoTracker Red DND-99 (LY) staining. Before live-cell imaging, cells were incubated for 2 h with 200 nM Baf A1. Representative images are shown. The lower panels depict enlargements of the boxed areas in the upper panels. Scale bars, 10 µm. insets: 2 µm.