Characterization of an inflammatory monocyte population enriched in Irf3 ^R278Q/R278Q mice. (A) Dot plot showing enriched GO terms in monocyte #1. (B–D) Dot plots showing expression in monocyte subpopulations of (B) transcripts for proteins involved in HSV-1 sensing; (C) HSV-1 RNA; and (D) selected transcripts for NF-κB–stimulated genes, IFNγ-induced genes, and ISGs. (E) Dot plot showing expression of Ifng across cell types annotated in the scRNAseq data set. (F) Illustration of setup for analysis of cell–cell communication between monocyte #1 and brain-resident cells using NicheNet. Image was generated in BioRender. (G–I) Dot plot showing enriched GO terms at the level of ligands and target cell functions from the NicheNet analysis of the communication between monocyte #1 and (G) neurons, (H) astrocytes, and (I) endothelial cells. Expression levels (B and D) are shown as average expression, and average count of expressing cells (C and E) and range color coded from blue (lowest) to yellow (highest). The hypergeometric distribution was employed to calculate P values for GO enrichment analysis. The false discovery rate (FDR) was subsequently applied to correct for multiple testing. Adjusted P value shown in color range (red [lowest] to blue [highest]). P values <0.05 were considered statistically significant.