Figure 2. Unbiased characterization of... | Rockefeller University Press

$Unbiased characterization of the LRRK2 lysosomal proteome. (A and B) Schematic depiction of the experimental workflow of the lysosomal immunoprecipitation (LYSO-IP) in HEK293T stably expressing LRRK2, reasoning that the proteins recruited by LRRK2 to damaged lysosomes will be absent if the cells are pre-treated with the LRRK2 kinase inhibitor MLi2. (C) Volcano plot showing the subset of proteins with a fold change >1.3 and P value <0.05 present on the lysosomal fraction of cells treated with LLOME vs LLOME+MLi-2. Data are from four independent experiments. (D) Gene Ontology (GO) search of the top five enriched terms for Biological Process of the 46 candidates found in C, with fold enrichment indicated on the horizontal axis. (E) The 46 candidate proteins were grouped by molecular function. (F) Model of the LRRK2 lysosomal proteome, where LRRK2 recruits nine RAB substrates and two RHD family members to damaged lysosomes.$

Figure 2.

Unbiased characterization of the LRRK2 lysosomal proteome. (A and B) Schematic depiction of the experimental workflow of the lysosomal immunoprecipitation (LYSO-IP) in HEK293T stably expressing LRRK2, reasoning that the proteins recruited by LRRK2 to damaged lysosomes will be absent if the cells are pre-treated with the LRRK2 kinase inhibitor MLi2. (C) Volcano plot showing the subset of proteins with a fold change >1.3 and P value <0.05 present on the lysosomal fraction of cells treated with LLOME vs LLOME+MLi-2. Data are from four independent experiments. (D) Gene Ontology (GO) search of the top five enriched terms for Biological Process of the 46 candidates found in C, with fold enrichment indicated on the horizontal axis. (E) The 46 candidate proteins were grouped by molecular function. (F) Model of the LRRK2 lysosomal proteome, where LRRK2 recruits nine RAB substrates and two RHD family members to damaged lysosomes.

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