LRRK2 recruitment to membrane damaged lysosomes. (A) U2OS cells were transfected with HaloTag-LRRK2 for 36 h and then imaged live after treatment with DMSO or LLOME (2 h). (B) U2OS cells transfected with HaloTag-LRRK2 and LAMP1-RFP for 36 h were treated with LLOME (2 h) and imaged live. (C) 3D view using “volume view” plugin from Fiji. (D) U2OS cells were transfected with HaloTag-LRRK2 and GFP-GAL3 for 36 h and treated with LLOME. (E) Graph shows the percentage of total LRRK2⁺ lysosomes that colocalize with GAL3 (yellow) and the percentage of total GAL3⁺ lysosomes that colocalize with LRRK2 (cyan) per cell. Data are mean ± SEM. n = 21 cells. Scale bar A = 10 µm; (B–E) = 1 µm. Insets (A) = 2 µm.