Condensates detection methods. (A) Microscopy methods used to observe condensates can be divided into two groups: Fluorescently labeled condensates can be directly observed by fluorescence microscopy. Other methods use physical properties of condensates to detect them without labeling. (B–D) Size-exclusion beads packed in a column or dispersed in the sample discriminate condensates based on their size. (C) Dynamic light scattering takes advantage of Rayleigh diffusion from particles in the medium to assess the particles size. (D) Proteins and particles migration speed in native PAGE vary accordingly with their size and charge. Figure created using Biorender (https://biorender.com/).