Condensate purification methods. (A) The six main ways to purify or enrich condensates: (A) Differential centrifugation postulates that condensates have a specific sedimentation coefficient range that allows their purification through several cycles of centrifugation. (B) Gradient centrifugation allows purification of condensates based on their specific densities. (C) FAPS takes advantage of flow cytometry to separate fluorescently tagged condensates from the rest of the lysate. (D) SEC separates the different particles based on their retention time on porous beads. (E) Tagged condensate proteins can be extracted from the rest of the lysate by antibodies grafted on beads. (F) Membrane filtration allows for quick sorting between condensate structures and smaller complexes. FAPS, fluorescence-activated particle sorting. Figure created using Biorender (https://biorender.com/).