Distinct early lung lesion composition in C57BL/6 and C3HeB/FeJ mice. (a) Additional representative lung lobe images of multiplex immunofluorescence analysis from each mouse at early time points after HN878 infection. Images from the mice at 14 and 21 days after infection shown in Fig. 5 a are also reproduced here. Scale bar = 1 mm. (b) Images showing individual fluorescent antibody staining corresponding to the images in Fig. 5 d. Scale bar = 50 μm. (c and d) CD4⁺ T cell numbers in whole left lung lobes from experiments shown in Figs. 5 and 6, respectively, normalized to tissue area. Data shown are from single experiments with N = 4–5 mice per group and are representative of two independent experiments. Plots show individual mice with lines at the mean. Statistical analysis: unpaired t test; ****, P < 0.0001.