Distinct early chemokine expression in lungs of C57BL/6 and C3HeB/FeJ mice. (a) Chord plots showing inferred interaction strength via the indicated receptor–ligand pairs between cell populations in the different conditions. Outer circle and arrow colors indicate the predicted sending population, and the inner circle colors indicate the predicted receiving populations. Arrow thickness indicates the overall signaling contribution. (b) Dot plots showing expression of the indicated genes in myeloid cell populations in scRNA-seq data. Circle sizes represent the abundance of cells expressing the gene, as a percentage of cells within the cluster. Circle color is proportional to the mean expression of the gene within all cells in the cluster. (c) Bar plots showing the relative contribution of the indicated receptor/ligand interactions to total inferred interaction activity in each group. Data in a–c are from a single scRNA-seq experiment, and plots show combined data from cells from N = 3 mice per group. (d) DESeq2-normalized expression values of the indicated chemokine genes in whole lung. Data shown are from individual mice from a single bulk RNA-seq experiment with N = 5 mice per group and lines at the mean. Statistical analysis: two-way ANOVA with Holm–Sidak post hoc test: *, P < 0.05; ***, P < 0.001; ****, P < 0.0001; ns, not significant.