HI Lachnospiraceae species do not affect intratumoral butyrate concentration. GF C57BL/6 mice (n = 5/group) were fed HI Lachnospiraceae and K. alysoides (TSD-26) weekly for 1 mo. 10⁸ CFU of TSD-26 were HI at 100°C for 30 min and fed to one mouse. The control group was fed the same volume of autoclaved water. Then the mice were injected s.c. with 10⁶ MC38 cells. On day 10 after tumor inoculation, tumors (control group, n = 14; HI-TSD-26 group, n = 10) were collected for butyrate detection by GC/MS. Unpaired t tests were used to analyze the data. n.s., not significant. Data were pooled based on two experiments yielding similar results.