Figure 7.
Tight coordination between ribosomal and mitochondrial components in human cells and implications of RPS10 reduction on mitochondrial activity. (A) Proportionality across all genes was calculated based on 13 human cell lines derived from different individuals (Cenik et al., 2015). Rho represents a measure of proportionality, indicating how changes in one gene’s expression are proportionate to changes in another. The significant proportional relationships (rho >0.75) between ribosomal and mitochondrial membrane genes were identified both at the RNA level (top left) and at the translation efficiency level (TE) (bottom left). These interactions were visualized by line interaction maps and compared to an unrelated dataset (cilia genes) to assess specificity. The number of interactions is displayed at the bottom for RNA and translation efficiency values. Ribosome genes are shown in pink, mitochondrial membrane genes are in green and cilia genes are colored in gray. (B) Normalized MitoTracker intensities in response to ∼50% reduction of RPS10, RPL35A, RPL5, and RPS23 transcripts were plotted in comparison to nontarget siRNA. Each gene specific RNAi data was normalized to the median of non-target RNAi control. MitoTracker intensity was quantified for at least 100 cells in three biological replicates, and RNA levels were quantified by quantitative PCR for each replicate (Fig. S5 D). To evaluate significant differences between RP RNAi and wild-type controls, P values were calculated using a two-tailed Student’s t test. Data distribution was assumed to be normal but this was not formally tested. (C) ADP/ATP ratios in response to ∼50% knockdown of RPS10, RPL35A, RPL5, and RPS23 transcripts in comparison to non-target siRNA are shown. Each point represents the ADP/ATP ratio of a specific RP siRNA compared to non-target siRNA for each biological replicate. The bold line represents the median of the biological replicates. To assess differences in the ADP/ATP ratio between each RP RNAi and non-target RNAi, a paired two-tailed Student’s t test was performed. P values are displayed above each sample. Data distribution was assumed to be normal but this was not formally tested.

Tight coordination between ribosomal and mitochondrial components in human cells and implications of RPS10 reduction on mitochondrial activity. (A) Proportionality across all genes was calculated based on 13 human cell lines derived from different individuals (Cenik et al., 2015). Rho represents a measure of proportionality, indicating how changes in one gene’s expression are proportionate to changes in another. The significant proportional relationships (rho >0.75) between ribosomal and mitochondrial membrane genes were identified both at the RNA level (top left) and at the translation efficiency level (TE) (bottom left). These interactions were visualized by line interaction maps and compared to an unrelated dataset (cilia genes) to assess specificity. The number of interactions is displayed at the bottom for RNA and translation efficiency values. Ribosome genes are shown in pink, mitochondrial membrane genes are in green and cilia genes are colored in gray. (B) Normalized MitoTracker intensities in response to ∼50% reduction of RPS10, RPL35A, RPL5, and RPS23 transcripts were plotted in comparison to nontarget siRNA. Each gene specific RNAi data was normalized to the median of non-target RNAi control. MitoTracker intensity was quantified for at least 100 cells in three biological replicates, and RNA levels were quantified by quantitative PCR for each replicate (Fig. S5 D). To evaluate significant differences between RP RNAi and wild-type controls, P values were calculated using a two-tailed Student’s t test. Data distribution was assumed to be normal but this was not formally tested. (C) ADP/ATP ratios in response to ∼50% knockdown of RPS10, RPL35A, RPL5, and RPS23 transcripts in comparison to non-target siRNA are shown. Each point represents the ADP/ATP ratio of a specific RP siRNA compared to non-target siRNA for each biological replicate. The bold line represents the median of the biological replicates. To assess differences in the ADP/ATP ratio between each RP RNAi and non-target RNAi, a paired two-tailed Student’s t test was performed. P values are displayed above each sample. Data distribution was assumed to be normal but this was not formally tested.

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