Figure S2. Stress responses in RP... | Rockefeller University Press

Figure S2.

$Stress responses in RP haploinsufficient mutants. (A) Survival fractions analyzed after 8 h of acute oxidative stress after pretreatment of rapamycin, bortezomib, and cycloheximide in wild-type animals are shown (left). Wild-type animals pretreated with double combinations of rapamycin, bortezomib, and cycloheximide and survival fraction within 8 h of acute oxidative stress was determined (right). (B) Survival fractions analyzed after 8 h of acute oxidative stress after pretreatment of rapamycin, bortezomib, and cycloheximide in rpl-5(0)/+ mutants are shown. In A and B each point represents a biological replicate and bars represent the median of all biological replicates. Statistical analysis was performed using Log-rank test with Bonferroni correction. P values are provided on the top of the plot. For the right plot in A all samples were compared to no drug control. The “n = ” on the graphs or legends indicates the total number of animals analyzed. (C) An acute oxidative stress survival time course for skn-1(zj15) and daf-16(mu86), alongside wild-type animals, is shown. skn-1(zj15) and daf-16(mu86) animals are comparable in acute oxidative stress survival compared to wild-type animals (P values are 0.52 and 0.08 for skn-1(zj15) and daf-16(mu86) respectively). (P = 0.85 for comparison of skn-1(zj15) versus daf-16(mu86)). P values were obtained using a Log-rank test with Bonferroni correction to compare the survival distributions of two or more groups for three biological replicates. (D) Density plots of log2 RNA expression differences of HSF-1 targets across all RP haploinsufficient mutants were plotted. HSF-1 targets are the significantly overexpressed genes in response to HSF-1 overexpression (Sural et al., 2019). (E) Acute heat stress survival was assessed in RP mutants, balancer controls, and daf-2(e1370) heat-stress-tolerant mutants after 2 h at 37°C. Statistical analyses were performed using Log-rank test, with Bonferroni correction for multiple samples. All experiments were conducted in three biological replicates, all animals were grown at 16°C. “n” in A–C represents the number of animals. Superscript numbers denote the specific balancers compared between an RP mutant and its wild type counterpart. Balancer chromosomes are denoted as follows: +1 = tmC20, +2 = tmC5, +3 = mIn1.$

Stress responses in RP haploinsufficient mutants. (A) Survival fractions analyzed after 8 h of acute oxidative stress after pretreatment of rapamycin, bortezomib, and cycloheximide in wild-type animals are shown (left). Wild-type animals pretreated with double combinations of rapamycin, bortezomib, and cycloheximide and survival fraction within 8 h of acute oxidative stress was determined (right). (B) Survival fractions analyzed after 8 h of acute oxidative stress after pretreatment of rapamycin, bortezomib, and cycloheximide in rpl-5(0)/+ mutants are shown. In A and B each point represents a biological replicate and bars represent the median of all biological replicates. Statistical analysis was performed using Log-rank test with Bonferroni correction. P values are provided on the top of the plot. For the right plot in A all samples were compared to no drug control. The “n = ” on the graphs or legends indicates the total number of animals analyzed. (C) An acute oxidative stress survival time course for skn-1(zj15) and daf-16(mu86), alongside wild-type animals, is shown. skn-1(zj15) and daf-16(mu86) animals are comparable in acute oxidative stress survival compared to wild-type animals (P values are 0.52 and 0.08 for skn-1(zj15) and daf-16(mu86) respectively). (P = 0.85 for comparison of skn-1(zj15) versus daf-16(mu86)). P values were obtained using a Log-rank test with Bonferroni correction to compare the survival distributions of two or more groups for three biological replicates. (D) Density plots of log₂ RNA expression differences of HSF-1 targets across all RP haploinsufficient mutants were plotted. HSF-1 targets are the significantly overexpressed genes in response to HSF-1 overexpression (Sural et al., 2019). (E) Acute heat stress survival was assessed in RP mutants, balancer controls, and daf-2(e1370) heat-stress-tolerant mutants after 2 h at 37°C. Statistical analyses were performed using Log-rank test, with Bonferroni correction for multiple samples. All experiments were conducted in three biological replicates, all animals were grown at 16°C. “n” in A–C represents the number of animals. Superscript numbers denote the specific balancers compared between an RP mutant and its wild type counterpart. Balancer chromosomes are denoted as follows: +¹ = tmC20, +² = tmC5, +³ = mIn1.