PA biosensors can associate with the PM under resting conditions. (A–C) Basal localization of PILS-Nir1 (A), NES-PABDx2-Spo20 (B), and PASS (C). Each small symbol represents the biosensor intensity PM/Cyto ratio of a single cell at time 0 min, before any treatment was added (n = 418 PILS-Nir1 cells, 288 NES-PABDx2-Spo20 cells, and 74 PASS cells). The large symbols show the grand means of each experimental replicate (n = 3–6 independent experiments). The symbols are color-coded according to the figure where the data can be found. Pink cells are in Fig. 1. Blue cells are in Fig. 2. Green cells are in Fig. 7. Brown cells are cells co-expressing FKBP-PJ-Dead (a catalytically dead PIP phosphatase used as a control in Fig. 3). The shape of the symbol denotes different dishes within each experiment (i.e., cells that were to be treated with PMA or cells that were to be treated with PMA + FIPI). Note that not all treatments shown here were included in their respective figures. Error bars show the mean ± SEM. The gray shaded area shows the PILS-Nir1 grand mean ± SEM to facilitate comparison between graphs. (D and E) Representative confocal images of PILS-Nir1 (D) and NES-PABDx2-Spo20 (E) show the range of basal PM/Cyto ratios seen across these experiments, with the given ratio values labeled on each image. Source data are available for this figure: SourceData FS2.
Figure S2.

PA biosensors can associate with the PM under resting conditions. (A–C) Basal localization of PILS-Nir1 (A), NES-PABDx2-Spo20 (B), and PASS (C). Each small symbol represents the biosensor intensity PM/Cyto ratio of a single cell at time 0 min, before any treatment was added (n = 418 PILS-Nir1 cells, 288 NES-PABDx2-Spo20 cells, and 74 PASS cells). The large symbols show the grand means of each experimental replicate (n = 3–6 independent experiments). The symbols are color-coded according to the figure where the data can be found. Pink cells are in Fig. 1. Blue cells are in Fig. 2. Green cells are in Fig. 7. Brown cells are cells co-expressing FKBP-PJ-Dead (a catalytically dead PIP phosphatase used as a control in Fig. 3). The shape of the symbol denotes different dishes within each experiment (i.e., cells that were to be treated with PMA or cells that were to be treated with PMA + FIPI). Note that not all treatments shown here were included in their respective figures. Error bars show the mean ± SEM. The gray shaded area shows the PILS-Nir1 grand mean ± SEM to facilitate comparison between graphs. (D and E) Representative confocal images of PILS-Nir1 (D) and NES-PABDx2-Spo20 (E) show the range of basal PM/Cyto ratios seen across these experiments, with the given ratio values labeled on each image. Source data are available for this figure: SourceData FS2.

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