PILS-Nir1 does not aggregate or unfold in in vitro liposome experiments. (A) Schematic of the PILS-Nir1 liposome flotation assay. (B) Representative SDS-PAGE gel and quantification of PILS-Nir1 bound to liposomes in the liposome fraction (L), as soluble protein in the middle fraction (M), or as aggregated protein in the pellet fraction (P) after reacting with POPC liposomes of varying compositions. (C) CD for PILS-Nir1 with and without PA liposomes.