Additional data related to the scRNA-seq analysis of colonic Foxp3 ⁻ CD4 ⁺ T cells isolated from F1-SSP and uninfected hosts at 28 dpi. (A) Gating strategy used for sorting CD4⁺ T cells from colonic lamina propria of F1-SSP and ΔTreg SSP at 28 dpi. (B) UMAP plot showing the clustering of colonic CD4⁺ T cells based on condition (F1-SSP/ΔTreg SSP) at 28 dpi. (C) Barplot showing the contribution of each condition (F1-SSP/ΔTreg SSP) to the different CD4⁺ T cell clusters. (D) Heatmap representing the differential expression of the top 10 genes of CD4⁺ T cells generated in each condition (F1-SSP/ΔTreg SSP).