The WASH complex regulates the cell surface levels of SNX17- and SNX27-dependent cargoes. (A) DIV11 rat cortical neurons were infected with lentiviruses carrying ctrl- or WASHC5-shRNAs, and the surface levels of β1-integrin and GluA1 were determined at DIV17 using surface biotinylation assays. The total levels of β1-integrin, GluA1, and strumpellin were validated by western blot of the lysate, and GAPDH was used as a loading control. (B) The levels of surface β1-integrin protein were quantified and expressed as a percentage of ctrl-shRNA. ctrl-shRNA: 100%, VPS34-shRNA: 41.50 ± 8.006%. N = 3 independent experiments. Statistical significance was determined using unpaired two-tailed Student’s t test, **P < 0.01. Error bars are SEM. (C) The levels of surface GluA1 protein were quantified and expressed as a percentage of ctrl-shRNA. ctrl-shRNA: 100%, VPS34-shRNA: 51.02 ± 8.005%. N = 3 independent experiments. Statistical significance was determined using unpaired two-tailed Student’s t test, **P < 0.01. Error bars are SEM. DIV, days in vitro. Source data are available for this figure: SourceData FS8.