Phylogenetic analysis of eukaryotic pore-loop channels related to NALCN and Cch1. (A) Maximum likelihood phylogenetic tree of HMM-identified pore-loop channel protein sequences from a curated set of eukaryotic proteomes. Branches are colored according to taxonomic groupings as indicated by the legend. The chevrons with alphabetic labels (cyan colored) denote strongly supported nodes that separate NALCN/Cch1 (clade A), CaV1/CaV2 (clade B), CaV3 (clade C), and NaV (clade D) and associated eukaryotic channels from each other. (B) Maximum likelihood phylogenetic tree of select metazoan pore-loop channels and Cch1 homologues identified from an expanded set of fungal proteomes from the FungiDB database. Leaves on the tree are colored according to major taxonomic groupings within fungi. For both trees, node support values for three separate analyses, SH-aLRT, LBP, and aBayes, are depicted by circular symbols with colors reflecting ranges of values as indicated in the node support legend. SH-aLRT, Shimodaira–Hasegawa approximate likelihood ratio tests; aBayes, approximate Bayes tests; LBP, local bootstrap probability tests.