Cluster map of identified eukaryotic pore-loop channels with ≥18 predicted transmembrane helices. Edges correspond to BLAST comparison expect-values (E-values) colored according to the provided legend on the lower right of the map. Individual ion channel sequences are depicted by symbols that are colored according to the taxonomic groups indicated by the legend on the left of the plot. Metazoan NALCN, Na_V, and calcium (Ca_V) channels, as well as fungal Cch1 channels, are labeled.