Single-cell TCR repertoire analysis across blood, lymph node, and colon. (A) Upper panels: UMAP plots showing the annotation of CD4⁺ T cells isolated from inflamed colon, mildly inflamed colon, MLN, and peripheral blood of CD patients (n = 3, CD Patient 10–12 in Table S1). Lower three panels: Top six CD4⁺ T cell clones within T_RM_2 shared across different tissues in each patient. (B) Venn diagram showing the total number of shared clones across tissues. (C) Number of clones shared with T_RM_2 of inflamed and mildly inflamed colon in each CD4⁺ T cell cluster. Clusters sharing more than five clones are displayed.