Figure S2.
Genetic manipulation of mitochondrial dynamics in vivo alters mitochondria number and size. (A) Representative images (left column) and Imaris reconstructions (right column) of V1 P21 astrocyte mitochondria expressing EGFP (green) and shRNA against Drp1 (shDrp1), Mfn1 (shMfn1), Miro1 (shMiro1), or scrambled control (shControl). Scale bar, 10 µm. (B–D) Quantification of astrocyte average mitochondrial number (left graphs) and average mitochondrial size (right graphs) in shDrp1, shMfn1, and shMiro1 conditions, respectively. N = 4–5 male and female mice/condition (large circles), n = 2–4 cells/mouse, 10–15 cells total/condition (small gray dots). Data are mean ± SEM. Nested t test. (E) Representative images of Imaris reconstructed V1 P21 astrocytes expressing mCherry-CAAX–tagged shRNA (magenta) against Drp1 (shDrp1), Mfn1 (shMfn1), Miro1 (shMiro1), or scrambled control (shControl) and their EGFP mitochondria (green). Scale bar, 10 µm. (F–H) Quantification of total mitochondrial volume normalized to cell volume in (F) shDrp1, (G) shMfn1, and (H) shMiro1 conditions, respectively. N = 4–5 male and female mice/condition (large circles), n = 2–4 cells/mouse, 10–15 cells total/condition (small gray dots). Data are mean ± SEM. Nested t test.

Genetic manipulation of mitochondrial dynamics in vivo alters mitochondria number and size. (A) Representative images (left column) and Imaris reconstructions (right column) of V1 P21 astrocyte mitochondria expressing EGFP (green) and shRNA against Drp1 (shDrp1), Mfn1 (shMfn1), Miro1 (shMiro1), or scrambled control (shControl). Scale bar, 10 µm. (B–D) Quantification of astrocyte average mitochondrial number (left graphs) and average mitochondrial size (right graphs) in shDrp1, shMfn1, and shMiro1 conditions, respectively. N = 4–5 male and female mice/condition (large circles), n = 2–4 cells/mouse, 10–15 cells total/condition (small gray dots). Data are mean ± SEM. Nested t test. (E) Representative images of Imaris reconstructed V1 P21 astrocytes expressing mCherry-CAAX–tagged shRNA (magenta) against Drp1 (shDrp1), Mfn1 (shMfn1), Miro1 (shMiro1), or scrambled control (shControl) and their EGFP mitochondria (green). Scale bar, 10 µm. (F–H) Quantification of total mitochondrial volume normalized to cell volume in (F) shDrp1, (G) shMfn1, and (H) shMiro1 conditions, respectively. N = 4–5 male and female mice/condition (large circles), n = 2–4 cells/mouse, 10–15 cells total/condition (small gray dots). Data are mean ± SEM. Nested t test.

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