Validation of shRNA tools. (A) Schematic of shDrp1 sequence aligned to the mouse, rat, and human genomes. (B) Drp1 relative mRNA expression in control and shDrp1-transfected mouse 3T3 cells. (C) Schematic of shMiro1 and shMfn1 sequences aligned to the mouse genome. (D and E) Miro1 and (E) Mfn1 relative mRNA expression in control and shDrp1-transfected mouse 3T3 cells. Data are mean ± SEM. n = 3 independent experiments. Unpaired two-tailed t test.