BAL-0028 inhibits primate NLRP3 but is a poor inhibitor of NLRP3 from other mammals. Comparison of BAL-0028 and MCC950 in IL-1β release assays from cells stimulated with LPS and nigericin. (A–I) J774A.1 mouse macrophage cell line (A), Wistar rat PBMCs (B), Beagle CD14⁺ monocytes (C), New Zealand white rabbit PBMCs (D), African green monkey (C. sabaeus) PBMCs (E) and CD14⁺ monocytes (F), cynomolgus monkey (M. fascicularis) CD14⁺ monocytes (G), WT 129S6 iBMDM (H), and 129S6-human promoter NLRP3 iBMDM (I). (A, H, and I) Graph symbols show average IL-1β values relative to vehicle control ± SEM from N = 3 independent experiments performed in triplicate. (B–G) Graph symbols show average IL-1β values relative to vehicle control ± SD from one experiment performed in duplicate (C, D, and F) or triplicate (B, E, and G). IC₅₀ curves were fitted by nonlinear regression analysis.