BAL-0028 does not inhibit inflammasome-independent cytokine release and is not cytotoxic . Related to Fig. 1. (A and B) Effect of BAL-0028 and MCC950 on LDH release (A) and TNF release (B) from LPS- and nigericin-stimulated iMacs. (C and D) Comparison of BAL-0028 and MCC950 pre-treatment in PMA-differentiated THP-1s on LPS-induced secretion of (C) TNF and (D) IL-6. (E and F) Effect of BAL-0028 and MCC950 treatment in PMA-differentiated THP-1s on (E) cytotoxicity (LDH release) (F) and cell viability (CellTiter-Blue assay). (A–F) Bar chart symbols show average values relative to vehicle control from independent experiments (indicated by different symbols) performed in triplicate ± SEM. N = 3 (A, B, E, and F) and N = 2 (C and D).