Knockdown of TRIP6/CCT7 does not affect AMPK signaling. (A) Representative fluorescence images of WT or ATG7 KO HeLa cells stably expressing TFEB::mNG, transfected with siLuc, siRagC/D, or siFLCN. Arrowheads indicate nuclear-localized TFEB::mNG. (B) Quantification of image data shown in A (n = 3 biologically independent samples). (C) Representative immunoblots of TFEB, AMPK, p-AMPK, and GAPDH in WT or ATG7 KO HeLa cells, transfected with siLuc, siCCT7, or siTRIP6. (B) P values were determined by ANOVA with Tukey’s multiple comparison test; *P < 0.05, **P < 0.01. Source data are available for this figure: SourceData FS6.
Figure S6.

Knockdown of TRIP6/CCT7 does not affect AMPK signaling. (A) Representative fluorescence images of WT or ATG7 KO HeLa cells stably expressing TFEB::mNG, transfected with siLuc, siRagC/D, or siFLCN. Arrowheads indicate nuclear-localized TFEB::mNG. (B) Quantification of image data shown in A (n = 3 biologically independent samples). (C) Representative immunoblots of TFEB, AMPK, p-AMPK, and GAPDH in WT or ATG7 KO HeLa cells, transfected with siLuc, siCCT7, or siTRIP6. (B) P values were determined by ANOVA with Tukey’s multiple comparison test; *P < 0.05, **P < 0.01. Source data are available for this figure: SourceData FS6.

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