Super-resolution of granules at coverslip interface show no difference in granule number or granule area with liprin-α1 knockdown or with glucose stimulation. (A) STED Immunofluorescence images of insulin granules at the β cell–ECM interface resolved individual granules. Counts across a 25 × 25 µm area showed >1,000 granules in control cells and similar numbers (n = 3 animals, Student's t test, P = 0.15) and similar average area (n = 3 animals, Student's t test, P = 0.35) in liprin-α1 knockdown cells. (B) Using an identical approach and analysis to that in A counts across a 25 × 25 µm area showed >1,000 granules in control cells and similar numbers (n = 3 animals, Student’s t test, P = 0.35) and similar average area (n = 3 animals, Student’s t test, P = 0.64) in cells after 15 min stimulation with 16.7 mM glucose.