GFP-liprin-α1 clusters are the same size and density as endogenous liprin-α1 clusters. (A) Immunofluorescence staining of endogenous liprin-α1 in isolated β cells (grown on laminin-511). Liprin-α1 forms punctate spots/clusters across the β cell/laminin interface, as seen in the line scan (yellow) across a single β cell. (B) Live-cell super-resolution microscopy in β cells expressing GFP–liprin-α1 revealed similar punctate GFP–liprin-α1 spots/clusters, seen in the line scan (yellow) across a single β cell. (C and D) Characterization of GFP–liprin-α1 and native liprin-α1–immunostained cluster size (Student's t test P = 0.18, >139 clusters, 9 cells, 3 animals) and density across (Student's t test P = 0.06, 8–9 cells in each condition, n = 3 animals) the β cell–ECM interface.